Fans on five continents will be able to watch the concert live as it is played.
It's the first time a show of this size will be streamed live, U2 and YouTube claimed in their announcements of the event on Monday.
The show, the second-last the Dublin band will do this year in its 360 Degree tour, is expected to draw 96,000 fans to the Rose Bowl.
The live-streamed event, starting at 8.30 PT on Oct. 25, will be available in 16 countries, including the U.S., U.K. and Canada.
The full concert will be streamed on the U2 website. After it is over, fans can watch it again on the U2 website or on YouTube.
"A live stream of this magnitude gives us the opportunity to extend the platform and engage millions of people," YouTube spokesman Spencer Cross said.
YouTube would not say how many users it was expecting, but said it believed the network would be able to handle the traffic.
The video sharing service, which first ventured into live events with a concert broadcast in November 2008, said it is seeking out more live-streaming partnerships.
A Twitter feed where fans can share their experiences and a donation portal for U2 charity Red will accompany the concert.